FastQCFastQC Report
Fri 31 May 2024
EGAF00005488995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5489741
Total Bases554.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC219740.40027389270277053No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG198770.3620753693115941No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG192220.35014402318797916No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG170240.31010570444033697No Hit
GTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGC167450.3050234974655453No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC154280.2810332946490554No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA151710.27635183517765227No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT148180.2699216593278262No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT136920.24941067347257367No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT121470.22126726925732926No Hit
GGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGAT120740.21993751617790347No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC113850.2073868330035971No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT108780.19815142463005084No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC108090.19689453473305937No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC107590.19598374495263077No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC107020.1949454446029421No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG103480.18849705295750746No Hit
GCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACG103150.18789593170242458No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC102180.18612899952839304No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT96300.17541811171055247No Hit
GCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTA94400.1719571105449237No Hit
GGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGT91610.166874903570132No Hit
GGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCT84180.1533405674329627No Hit
GTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGG82660.15057176650045967No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT81440.14834943943621384No Hit
GTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAA80080.145872091233448No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCT79670.14512524361349652No Hit
GCCCGCTCTGTTTTCCCTGAACCAGGAGTCTCCAGATGTCAACACAGAAG74340.1354162245541274No Hit
GCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGGGGCATGGTGGGTTT74170.1351065560287817No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG73870.1345600821605245No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA72990.13295709214697013No Hit
GGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTG69610.12680015323127267No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT69490.1265815636839698No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT68800.1253246737869783No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC67560.12306591513151531No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG63960.11650822871242925No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC62860.11450449119548628No Hit
GTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTG62840.11446805960426912No Hit
CCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACC61980.11290150118193189No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT61220.11151710071568038No Hit
TTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAA59340.10809253114126877No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT58760.10703601499597157No Hit
TGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCG58170.10596128305506582No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG57690.10508692486585433No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC57600.10492298270537717No Hit
GGCACGGTCACCACGCTGCTGAGGGAGTAGAGTCCTGAGGACTGTAGGAC57060.10393932974251427No Hit
GCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGA56710.10330177689621423No Hit
CCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAG56010.10202667120361415No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC55960.1019355922255713No Hit
AGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCC55350.10082442869344838No Hit
GTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGG55280.10069691812418836No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT55070.10031438641640836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)