FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239683
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC40450.3262930926696583No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC34180.27571564666128356No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC28850.2327207842650097No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA28020.22602552426709085No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC27400.22102424571442864No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT26100.21053769391045934No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT25110.20255178138282123No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC24280.19585652138490242No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT24050.19400120837343096No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC23720.19133923753088491No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA23690.19109724018156254No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC23370.18851593512212395No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT23260.18762861150794197No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA23090.1862572931951152No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC21440.17294743898238502No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA21090.17012413657362405No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG20900.1685914866945824No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT20380.16439686597299472No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT19990.16125090043180396No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT19940.16084757151626666No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC19580.15794360332439825No Hit
ACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACT18380.14826370935150357No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC18320.14777971465285883No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA18170.146569727906247No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC17490.14108445465494002No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG17180.1385838153786089No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC17020.1372931628488896No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC16660.13438919465702118No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA16470.13285654477797953No Hit
AGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAA15970.12882325562260674No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG15940.1285812582732844No Hit
CTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGGTG15270.12317665080508483No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC15010.12107934044429101No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC14790.11930469321592699No Hit
GGTGAAATGAAAGTGCCAGATGTTGACATCAGAGGGCCCAAAGTGGACAT14790.11930469321592699No Hit
GGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGC14690.11849803538485243No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG14570.11753004598756295No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC14270.11511007249433929No Hit
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG14260.11502940671123181No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG14020.11309342791665287No Hit
GGTGAATTGGCTTATTTTGTCTGTCCCTGTCTGAATGTATTGCAGGAATT13980.11277076478422306No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC13900.11212543851936344No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT13900.11212543851936344No Hit
GTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCC13540.10922147032749502No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT13410.10817281514709809No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAG13310.10736615731602353No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC13220.10664016526805643No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC13200.10647883370184152No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA13160.1061561705694117No Hit
CTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACC13080.10551084430455206No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG13050.10526884695522967No Hit
CTCTCACATTGTCCTGTTCTCATGCTGAGGAGCCTGAGATCCCTGTGTGG12680.10228421298025382No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG12440.10034823418567489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAT1703.6379788E-1233.5353058
GCCAGTA1850.033.3964584
AACACGT1102.7299156E-630.2325868
TAGCATA2151.8189894E-1228.7248129
TAACACG1205.417336E-627.685087
CGACAAT350.00838786727.14652474-75
TAGTGCG350.00840283827.13660478-79
TATACTA1151.2764166E-424.7980483
ATACTAA1151.2771213E-424.7960324
AGGGTAC1401.7710558E-523.7647952
GTCTAAC1801.94108E-723.7628654
CATATAC1401.7727281E-523.7618981
GCGCTAT500.001652879623.7532182-83
CTCGATT2450.023.2684548-49
TCACCCG3950.022.8623353
CCGACAT1253.8380676E-1022.80215590-91
CCAGTAG2554.8930815E-1022.3595945
CGGCCCG4250.022.356878
ATAGCTC2550.022.34597438-39
TCTCGAT2450.022.29893148-49
Produced by FastQC (version 0.11.5)