FastQCFastQC Report
Fri 31 May 2024
EGAF00005489006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005489006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091785
Total Bases110.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT40220.3683875488305848No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT35290.32323213819570706No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC32820.3006086363157581No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC32350.29630375943981646No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA30050.27523734068520816No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT28360.2597581025568221No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT28300.2592085438067019No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC27560.2524306525552192No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC27280.2498660450546582No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA26820.24565276130373653No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC26570.24336293317823565No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC26160.23960761505241418No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA26060.2386916838022138No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG24200.22165536254848708No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC23910.21899916192290605No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC23070.21130533942122304No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG21980.20132168879403914No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC21840.20003938504375862No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG21400.19600928754287703No Hit
GGAAAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGG20500.1877659062910738No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT19660.18007208378939077No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT19440.17805703503894998No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA18870.17283622691280792No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG18780.1720118887876276No Hit
ATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTAT18680.17109595753742726No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT18260.1672490462865857No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG17750.1625777969105639No Hit
GTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAA17250.15799814065956208No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG16880.15460919503382076No Hit
GAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCT16800.1538764500336605No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT16640.1524109600333399No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA15770.14444235815659678No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC15760.14435076503157673No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT15320.14032066753069514No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC15190.13912995690543467No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT14970.1371149081549939No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC14600.13372596252925256No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG14420.1320772862788919No Hit
GTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCC14100.12914630627825074No Hit
GGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCT14080.12896312002821067No Hit
AAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGA13960.12786400252797026No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAG13680.12529939502740925No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA13650.12502461565234915No Hit
ACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACT13650.12502461565234915No Hit
CAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCAC13500.1236507187770486No Hit
CTTGGCTGTTGACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAG13500.1236507187770486No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCT13490.12355912565202856No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC13420.1229179737768883No Hit
GGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGG13290.12172726315162784No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT13150.12044495940134733No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC13110.12007858690126719No Hit
GACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCC13040.11943743502612694No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT12960.11870469002596665No Hit
TGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGG12650.11586530315034554No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG12460.11412503377496484No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA12320.11284273002468434No Hit
GATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAA12270.11238476439958417No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGC12250.1122015781495441No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG12230.11201839189950404No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT12020.11009493627408327No Hit
CCTAGTTGGGGCTCTGTTGTGTCTGATTTCCCCTGGCTTATTCTTTACTT12000.10991175002404319No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC11840.10844626002372262No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC11790.10798829439862244No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC11760.10771351502356233No Hit
TGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCC11690.10707236314842208No Hit
CCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAG11680.10698077002340203No Hit
CTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGT11650.10670599064834194No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC11570.10597324564818164No Hit
GGTGAATTGGCTTATTTTGTCTGTCCCTGTCTGAATGTATTGCAGGAATT11390.10432456939782099No Hit
AGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAG11330.10377501064770078No Hit
AGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGG11310.10359182439766072No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC11290.10340863814762064No Hit
GTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCCC11280.10331704502260061No Hit
GGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCAT11280.10331704502260061No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC11200.10258430002244032No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG11170.10230952064738022No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT11030.10102721689709972No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA11020.10093562377207967No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC10950.10029447189693942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)