FastQCFastQC Report
Fri 31 May 2024
EGAF00005489024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005489024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706317
Total Bases172.3 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA53340.31260310950427145No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG44590.261323071855933No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT39840.2334853371325492No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC36520.21402822570483682No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT34130.20002144970717634No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG33380.1956260179087473No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC32130.18830029824469896No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG31720.1858974621948911No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31200.182849962814647No Hit
ATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTAT28950.16966366741935995No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC27340.16022814049206566No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT27310.1600523232201285No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA27130.15899741958850555No Hit
CTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGT26470.15512943960588801No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA25970.15219915174026866No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC25390.1488000178161502No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC24870.14575251843590611No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC24630.14434598026040882No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT24460.14334968238609824No Hit
GTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCA23210.1360239627220499No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC22430.1314527136516837No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC22420.1313941078943713No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT22250.13039781002006076No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT21790.12770194518369096No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT21590.12652983003744322No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC21340.12506468610463353No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG21000.1230720903560124No Hit
AGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC20830.12207579248170182No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA20450.11984877370383112No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT20350.11926271613070724No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT20220.11850084128564621No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA20100.11779757219789756No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC19660.11521891887615256No Hit
TAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCT19610.11492589008959063No Hit
CTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTC19540.11451564978840392No Hit
GATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATG19390.1136365634287181No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC19330.11328492888484377No Hit
CAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCAC18970.11117512162159787No Hit
GTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAA18950.11105791010697309No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC18950.11105791010697309No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC18880.11064766980578639No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT18810.11023742950459967No Hit
GAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCT18640.10924113163028909No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT17990.10543175740498395No Hit
GATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTC17970.10531454589035917No Hit
ATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCCCTAC17810.10437685377336098No Hit
GTCCTTTTTCTTTTTAAACAGTTCCTTATGTTAGCCATGAAATCTAGCTG17680.10361497892829995No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTG17530.10273589256861416No Hit
GCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGC17490.10250146953936462No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC17420.1020912292381779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)