FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005489026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005489026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775689
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC85870.22742868917434672No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC74710.1978711700036735No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT61100.1618247689362127No Hit
AGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTG56600.149906414431909No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC56560.14980047350298184No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT54410.14410614857314785No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT54150.1434175325351214No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC54030.14309970974833996No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA52470.13896801352018134No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC51040.13518062531103595No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG49670.13155214849528127No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT46660.12358009359351367No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT45800.12130236362158006No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA44990.11915705981080538No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC43960.11642908089093142No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA42590.11280060407517674No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT39040.10339834663289268No Hit
AGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAGGTGGGATGCAGATCT38600.1022329964146941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA11450.048.5247159
CCAGTAG13350.043.004045
GTAGCAT13200.042.815018
GCCAGTA13850.040.4255374
CAGTAGC14100.040.0959936
TGCCAGT16800.033.3270073
GTCTAAC7450.031.479611
TAACACG8000.029.9088544
TCACCCG23100.029.6240063
CTCACCC24250.027.8244842
CCTCACC28500.024.5187051
CTCGATT16800.024.32429348-49
TCTAACA9700.024.1737542
TCAGACG6700.023.5699622
ATAGCTC17450.023.25764738-39
ACCCGGC29250.023.2258575
CACCCGG30000.022.9699974
ACGTGCG9950.022.9108478
CTAACAC10250.022.8766253
CGGACAG18300.022.82750318-19
Produced by FastQC (version 0.11.5)