FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005489035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005489035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816394
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC56200.6883931043099287No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA51550.6314353118714738No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA51260.6278831054613336No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG49480.6060799074956454No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG36340.4451282101534308No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG34010.4165880689960975No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA32920.4032366724890188No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG32150.3938049520207155No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG30690.37592143009380274No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC27300.3343973620580259No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC26680.32680298973289856No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC25820.31626886037868973No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT25670.31443151223551374No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC25130.30781705892008027No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT23530.28821867872620327No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT23460.28736124959272114No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC22870.28013434689622907No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG22690.2779295291244179No Hit
ATCCACAATTGCTGAAAGTCACCTGGGGCATGGTGGGTTTTGATCTTCTT22530.27596969110503017No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT21380.2618833553406811No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC20440.25036930697677834No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT20430.2502468171005667No Hit
AGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAA20340.24914440821466105No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC20230.247797019576332No Hit
GGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACA20130.2465721208142147No Hit
GCCCGCTCTGTTTTCCCTGAACCAGGAGTCTCCAGATGTCAACACAGAAG19950.24436730304240356No Hit
ATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAGG19810.24265244477543932No Hit
CACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGA19800.24252995489922757No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT19690.24118256626089854No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA18940.2319958255450187No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT18440.22587133173443216No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC18330.22452394309610313No Hit
GTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCC18060.2212167164383864No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC17880.21901189866657522No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG17690.2166845910185523No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT17480.21411230361810593No Hit
GTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTG17190.21056009720796576No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG17160.21019262757933058No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA17050.20884523894100152No Hit
GCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGG16960.20774283005509597No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC16730.20492556290222613No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT16160.19794363995815747No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG16130.19757617032952227No Hit
GCTCAAAGTTACCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTG15410.18875689924227762No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT15310.1875320004801603No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG15010.18385730419380839No Hit
CCTTGACATTCTCGATGGTGTCACTGGGCTCCACCTCGAGGGTGATGGTC14820.1815299965457855No Hit
CTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAAT14730.1804275876598799No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC14450.17699787112595144No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTT14350.17577297236383413No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT14230.17430309384929335No Hit
CCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC14120.17295570521096432No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTT13990.17136333682021182No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA13890.1701384380580945No Hit
GGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACA13880.17001594818188276No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT13710.16793362028628334No Hit
CCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAA13630.16695370127658948No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA13540.1658512923906839No Hit
GCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGGGGCATGGTGGGTTT13460.16487137338099006No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT13420.16438141387614313No Hit
CTCTTGTTTTCCCTGGACCTGGCATCTCCAGGTGTCAACACAGAATTAAG13400.16413643412371967No Hit
GGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAAC13330.16327900499023754No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA12960.1587468795704035No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG12900.15801194031313312No Hit
CAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGC12750.15617459216995716No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC12750.15617459216995716No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC12690.15543965291268677No Hit
CCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCA12470.15274487563602868No Hit
GCATCCACAATTGCTGAAAGTCACCTGGGGCATGGTGGGTTTTGATCTTC12390.15176495662633482No Hit
CGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTT12340.15115250724527618No Hit
CCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTT12160.148947689473465No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG11740.1438031146725723No Hit
CCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCA11280.1381685803668327No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGT11260.13792360061440923No Hit
CCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGA11220.1374336411095623No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC11220.1374336411095623No Hit
AGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGG11140.13645372209986845No Hit
GTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAA11090.1358412727188098No Hit
TCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCT10880.13326898531836343No Hit
CAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGC10820.13253404606109304No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG10790.13216657643245786No Hit
CTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG10520.12885934977474112No Hit
GGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTT10480.1283693902698942No Hit
CTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGAGT10340.12665453200292995No Hit
GGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTT10330.12653204212671823No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG10150.12432722435490706No Hit
GTCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC10140.12420473447869533No Hit
CTCCAATATGTAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT9980.12224489645930763No Hit
CATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAA9930.12163244707824898No Hit
CTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTT9900.12126497744961379No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT9890.12114248757340207No Hit
CATCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAG9860.12077501794476686No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC9780.11979509893507302No Hit
CCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT9580.1173453014108384No Hit
TCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC9480.11612040264872107No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG9370.11477301401039204No Hit
CCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCC9300.11391558487690992No Hit
GGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAAC9200.11269068611479262No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG8980.10999590883813452No Hit
GTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTGCTGAGCC8970.10987341896192279No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG8960.10975092908571107No Hit
GCTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGT8810.10791358094253509No Hit
CTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCT8800.10779109106632337No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG8790.10766860119011165No Hit
CTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATC8700.10656619230420607No Hit
CTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCC8580.10509631378966529No Hit
GTCGAATAACATCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTC8530.10448386440860663No Hit
CTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC8430.1032589656464893No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG8240.10093165799846644No Hit
TGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCT8220.10068667824604297No Hit
GCCTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT8170.10007422886498432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)