FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005489036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005489036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816394
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT46620.5710478028990904No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC45550.5579413861444351No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT36250.4440258012675252No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33040.4047065510035595No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC32120.3934374823920803No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC29090.3563230498999258No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA26180.320678495922312No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT26160.3204335161698886No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT25250.309286937434621No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA25240.3091644475584093No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA24520.30034517647116465No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG22360.27388736320943075No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC22240.27241748469488997No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC21740.2662929908843034No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT21100.2584536388067526No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC21080.25820865905432916No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC20930.25637131091115317No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA19620.2403251371274164No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC18980.23248578504986564No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA18230.2232990443339858No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA18080.22146169619080983No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC17850.21864442903794No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG17610.21570467200885848No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG17410.21325487448462382No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC15710.19243159552862957No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT15600.1910842068903005No Hit
GGAAAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGG15500.1898593081281832No Hit
AAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGA15400.1886344093660659No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC15260.18691955109910166No Hit
GGTGAATTGGCTTATTTTGTCTGTCCCTGTCTGAATGTATTGCAGGAATT15210.18630710171804302No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT15200.18618461184183127No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT14800.18128501679336204No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC14710.18018260790745644No Hit
ACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACT14340.1756504824876224No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC14330.17552799261141067No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC14320.17540550273519895No Hit
TGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGG14180.1736906444682347No Hit
GTTTCTGATTCCCCCTGGCTTATTCTTTACTTTTTCCTACTTTTCCAGGC13780.16879104941976544No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT13450.16474888350477832No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC13420.16438141387614313No Hit
GTTGTGTCTGATTTCCCCTGGCTTATTCTTTACTTTTTCCTCCTTTTCCA13420.16438141387614313No Hit
CTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAAAACATG13350.16352398474266103No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG13220.16193161635190853No Hit
AGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG12930.15837940994176833No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG12840.15727700105586273No Hit
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC12810.15690953142722755No Hit
GGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGC12620.15458222377920466No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC12550.15372479464572253No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT12200.14943764897831194No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT11970.14662038182544213No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC11810.14466054380605442No Hit
GTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAA11460.14037339813864383No Hit
CTTGGCTGTTGACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAG11420.13988343863379693No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA11260.13792360061440923No Hit
AGAAGAGGAAGAAGACCAAGACCCACCATGCCCCAGGCTCAGCAGGGAGC11150.13657621197608016No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG11040.13522882333775113No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT10910.13363645494699863No Hit
ATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTAT10860.13302400556593996No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG10830.13265653593730478No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT10710.131186657422764No Hit
CAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGA10550.1292268194033763No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAGGTTATCT10400.12738947126020034No Hit
GAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCT10380.12714449150777687No Hit
CTGTTGTGTCTGATTTCCCCTGGCTTATTCTTTACTTTTTCCTCCTTTTC10370.12702200163156516No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA10330.12653204212671823No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC10310.12628706237429477No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG10300.12616457249808305No Hit
CTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAA10260.1256746129932361No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA10250.12555212311702438No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT10240.12542963324081263No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT10190.12481718385975399No Hit
ATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAA10090.12359228509763669No Hit
GGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGAT9980.12224489645930763No Hit
CTGATTTCCCCTGGCTTATTCTTTACTTTTTCCTCCTTTTCCAGGCTCAG9950.12187742683067244No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG9890.12114248757340207No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG9850.12065252806855514No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA9780.11979509893507302No Hit
GTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAG9730.11918264955401436No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG9700.11881517992537918No Hit
CTCTCACATTGTCCTGTTCTCATGCTGAGGAGCCTGAGATCCCTGTGTGG9650.11820273054432053No Hit
TTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGG9640.11808024066810878No Hit
GATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAA9620.11783526091568532No Hit
GAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAA9470.11599791277250936No Hit
CATGGATGAAATTGAAAAGTACAAAGAAGTGGAAGAAGACCAAGACCCAT9360.1146505241341803No Hit
TGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCA9180.11244570636236915No Hit
GAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC9140.11195574685752221No Hit
CCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTT8960.10975092908571107No Hit
AGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAA8860.10852603032359374No Hit
CTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACC8740.10705615180905297No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC8530.10448386440860663No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCT8530.10448386440860663No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC8530.10448386440860663No Hit
CCGTCTCAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGA8440.10338145552270105No Hit
CTACAACATCCAGAAAGAGTCCACCTTGCACCTGGTGCTCCGTCTCAGAG8390.1027690061416424No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC8390.1027690061416424No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG8330.102034066884372No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG8320.10191157700816028No Hit
CTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGG8240.10093165799846644No Hit
GTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCA8230.1008091681222547No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT8190.10031920861740776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAG857.201379E-944.7169576
TCTATCG459.597006E-442.2457663
CGTGATC951.9197614E-840.0173458
GTAGGAT608.318848E-539.5588381
TAGGATT853.624791E-739.1370352
CCCGAAT1500.038.0141225
AAGCTTA905.670354E-736.9650424
CGCAGGT1800.036.921581
GATCCCC1750.035.2638781
TAACGCG1300.034.73089252-53
CTATCGT550.002572131634.5647164
CCGATCC1250.034.2095360-61
CGAATCC1753.6379788E-1232.5815167
TAAACCC1051.8924948E-631.6843243
GTACTTC600.003939495431.6843244
CTCGTAC600.00394069131.6823622
CCTCGTA1351.2034434E-831.647071
ACGCGAC1550.030.65560254-55
GCGACCG1550.030.65560256-57
CTTAAGG1102.7255355E-630.2366357
Produced by FastQC (version 0.11.5)