FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005489038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005489038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777222
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97980.35279858794147534No Hit
CCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTAC68300.24592920551543954No Hit
CCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGT57700.20776156893471245No Hit
GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGA52390.1886417434400275No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC50160.18061213687634622No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47000.16923386031077098No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC45990.16559713267430548No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC42670.15364274083958718No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC40480.1457571630931917No Hit
CCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGG38170.13743949889493892No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC32030.11533107544157435No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA30750.11072215328842994No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAAC29610.10661733199578571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC12850.045.0990837
AGCGCTC13050.044.0471089
GAACCAT29400.035.386649
TCACGTG17100.034.171148
AGGCAGC18400.034.079935
CAGCGCT17850.032.4692348
ACGGAAC5600.032.232266
GTCACGT17800.032.023757
CCCAGAG26250.032.013561
CCAGACG5750.031.402262
GGTCACG18400.031.2365266
AGGAGGC21050.029.5708892
CAGACGG6300.028.658713
GAGGGAA29850.028.4872975
GACGGAA6400.028.2063035
CACGTGC22700.027.6242589
CGGAACC6900.027.5373347
GGAACCA40100.026.1817288
CAGGAGG23700.026.042641
CGTAGCG9550.025.5694-95
Produced by FastQC (version 0.11.5)