FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14912919
Sequences flagged as poor quality0
Sequence length35-155
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG4260700.08.758298145-149
CGGGGGG648150.07.0351486145-149
GCGGGGG674550.06.686915145-149
GGGGCGG575150.06.474649145-149
GGGCGGG610100.06.413736145-149
GGCGGGG679300.06.219753145-149
GGGGGCG618800.05.962914145-149
TGGGGGG517200.05.9458456145-149
AGGGGGG292300.05.920302145-149
GACGCTT78050.05.79139141
CTATGCG64450.05.7884885
ACGCTTC72650.05.7276192
GGGGGGT348500.05.6168027145-149
CGATCGG19250.05.4036045145-149
GGTCGGG125550.05.2422066145-149
GATCGTA14507.8216544E-115.238168145-149
GGGTGGG611950.05.189032145-149
GATCGGT21900.05.1815386145-149
GTGGGGG664550.05.054368145-149
Produced by FastQC (version 0.11.5)