FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14912919
Sequences flagged as poor quality0
Sequence length35-155
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGCG67100.06.809448145-149
CGATCGG14400.06.239817145-149
TAGTCCG5604.0685874E-55.803599145-149
CGGGGGG230700.05.759353145-149
AGCGCGG73950.05.713347145-149
TAGAGCG30300.05.678536145-149
CCGATCG8052.6088146E-65.2247233145-149
GCGGGGG251850.05.177009145-149
CTATGCG61350.05.1479065
GCGCGTC48850.05.0484915145-149
Produced by FastQC (version 0.11.5)