FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19280553
Sequences flagged as poor quality0
Sequence length35-155
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG4906450.011.605607145-149
CGGGGGG709900.09.334974145-149
GCGGGGG738850.08.906385145-149
GGGGCGG648300.08.479853145-149
TGGGGGG597900.08.362313145-149
GGCGGGG759800.08.175507145-149
GGGCGGG714800.08.116619145-149
TATATCG24300.08.06463145-149
AGGGGGG345750.07.8680563145-149
GGGGGCG693500.07.7115107145-149
CTGAACG54800.07.481587145-149
GATCGGG87500.07.455721145-149
GGGGGGT410400.07.432836145-149
GGTCGGG155500.07.4123216145-149
ACGTGAT99550.07.382114145-149
ACAACGT105650.07.346393145-149
TCGGGGG159600.07.238062145-149
TAGCTCG36700.06.955787145-149
ACAACGG66900.06.899285145-149
GTCGGGG174350.06.7884073145-149
Produced by FastQC (version 0.11.5)