FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16592968
Sequences flagged as poor quality0
Sequence length35-155
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG4675750.011.72747145-149
TATATCG14850.09.10664145-149
CGGGGGG638300.09.01212145-149
GCGGGGG666500.08.9351635145-149
TGGGGGG555050.08.383096145-149
GGGGCGG579850.08.31824145-149
GGCGGGG661350.08.186819145-149
GGGCGGG615550.08.092119145-149
GATCGGG72850.07.975341145-149
CTGAACG49300.07.822843145-149
GGGGGGT382550.07.6331043145-149
GGGGGCG614300.07.541922145-149
AGGGGGG341200.07.457195145-149
GGGTGGG698500.06.9662204145-149
GGTGGGG768000.06.6684194145-149
ACACGTC175600.06.659353140-144
TCGGGGG151000.06.6008058145-149
CTATGCG166000.06.5996025
CGATCGG34200.06.590331145-149
GATCGGT29150.06.4433765145-149
Produced by FastQC (version 0.11.5)