FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16592968
Sequences flagged as poor quality0
Sequence length35-155
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGG112600.08.688886145-149
AAGCGCG147350.08.374772145-149
TAGCTCG37250.07.6551046145-149
TCGGGGG112800.07.5620546145-149
CGGGGGG367450.07.1381483145-149
CGCGTCG117350.07.017479145-149
GTCGGGG154700.07.007574145-149
TAGAGCG54550.06.9848385145-149
AGAGCGG361450.06.957398145-149
AGGGGGG273850.06.8670163145-149
GGTCGGG97350.06.792596145-149
GGGGCGG353450.06.6906223145-149
CGATCGG29650.06.6326137145-149
GATCGGT28200.06.62497145-149
TGGGGGG392100.06.56401145-149
GCGGGGG378450.06.56043145-149
GTCGGGT68950.06.517182140-144
TCGGGTA36550.06.5018373140-144
ATCGGAC31450.06.487492145-149
ACGAGCG41100.06.459536145-149
Produced by FastQC (version 0.11.5)