FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16990649
Sequences flagged as poor quality0
Sequence length35-155
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG3994350.011.037224145-149
CGGGGGG589950.09.245434145-149
GCGGGGG630850.08.786839145-149
CTGAACG50300.08.678938145-149
GGGGCGG542850.08.485998145-149
GATCGGG71900.08.330849145-149
TCACGCG123600.07.9263816145-149
GGGCGGG602400.07.920982145-149
TATATCG14350.07.6054106145-149
GGCGGGG650900.07.592028145-149
AGGGGGG294100.07.4908447145-149
TGGGGGG488600.07.449066145-149
GATCGGT31400.07.4364266145-149
CGAGTAA90200.07.372264145-149
GGGGGCG594350.07.36636145-149
GGTCGGG128750.06.9982142145-149
TCGTATG40800.06.9672914145-149
GGGGGGT328150.06.883728145-149
AGTCACG119250.06.78951145-149
CGCGTAA14250.06.7682257145-149
Produced by FastQC (version 0.11.5)