FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19104512
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15500.08.35689774
TCGTATG20550.07.20372274
CTCGTAT13151.44355E-86.472993473
ATAATCG7600.00456401245.84296464
CGAACTT43850.05.654757574
CGAACTA12801.5195146E-55.493411571
AATACGG13403.2594326E-55.24743871
TCTCGTA16802.0160678E-65.06660570
ACCGAAC65950.05.05040571
Produced by FastQC (version 0.11.5)