FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15533836
Sequences flagged as poor quality0
Sequence length35-155
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGG110850.08.657439145-149
AAGCGCG148650.08.286499145-149
TAGAGCG53200.07.9006443145-149
CGCGTCG112650.07.5485735145-149
CGATCGG30700.07.122496145-149
GATCGTA40350.07.0684395145-149
TCGGGTA37600.06.8089194140-144
CGGGGGG333800.06.747167145-149
GGGTCGG94300.06.6471596145-149
GTCGGGT75550.06.636181140-144
TAGCGCG15750.06.633091145-149
TAGCTCG33750.06.6228075145-149
GCGGGGG348150.06.6156116145-149
TCGGGGG103650.06.5163374145-149
AGATCGT82400.06.486696145-149
GATCGGT28200.06.4616017145-149
ATCGGAC32750.06.454105145-149
ACGAGCG39750.06.4177117145-149
CGGGTAG61600.06.4073114140-144
GTCGGGG145800.06.3821836145-149
Produced by FastQC (version 0.11.5)