FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106442
Sequences flagged as poor quality0
Sequence length80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCTTCATTGATTCCATCCGATTTCATTGGATGATGACTCCATTCGAG15040.1359312101312134No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCG450.003283786132.88547565
TATAACA951.9076897E-423.37127560
ATATAAC1002.699782E-422.20271159
CGAATAC950.00522741119.47606359
TAAATAC950.00523287819.4725446
CTTAGGC1401.2188485E-418.50058721
GAGACGG3051.6370905E-1118.1956544
TAGAGAC5450.017.6503412
GTATATC1050.00929811517.625981
ACGGGGT3002.5102054E-1017.2648757
GTAGAGA8500.016.5476861
CGGGGTT2953.741661E-916.3033928
CTACGAC1603.3850985E-416.1858267
TCGACGG1705.368158E-415.2337128
GTGCGAA2001.0934302E-414.79980274
AGAGACG3855.5479177E-1014.4147393
CTCGACG1808.280173E-414.3873967
GGTTATC1550.005089586614.3282161
GACTTAG1550.005103347414.32238819
TAGTCAG1600.006302758613.8735666
Produced by FastQC (version 0.11.5)