FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106442
Sequences flagged as poor quality0
Sequence length80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCTTCATTGATTCCATCCGATTTCATTGGATGATGACTCCATTCGAG13600.12291651979950147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12260.11080562740749175No Hit
AGTCCCTTCATTGATTCCATCCGATTTCATTGGATGATGACTCCATTCGA11370.10276182574414203No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT400.001841628337.00113363
ATGTCGC450.003283078832.8869173
ACGGACC708.8675914E-426.4293811
CGGACCT708.8675914E-426.4293812
CGCAATT708.869936E-426.42818330
TGCGATT1209.2655455E-418.49135872
GTAGAGA6950.017.5720651
TCTTCGG1450.003275820815.3101228
TAGAGAT4650.015.1180572
GGGGTTT4950.014.9492759
TAGTCTT1500.004105907414.79911224
AACGAGC2051.3480998E-414.44012148
GACGGAC2101.655108E-414.0956710
TGGGGTT3901.0253643E-813.2818578
GTTATTC1700.00940533813.0586317
TAGAGAC4607.90169E-912.0650412
TACTCAT4354.7093636E-811.9078714
GAGACGG2200.003745302311.7725554
GGACGGA2250.004429828411.51094159
TTCCCCG4053.0815117E-610.96329913
Produced by FastQC (version 0.11.5)