FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19577724
Sequences flagged as poor quality0
Sequence length80
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG3101.887016E-613.13194853
TTTTTAG244450.013.07554152
ATTTTTA266900.012.9325631
TTAGTAG229900.012.311055
TTTTAGT253150.012.2467843
TTTAGTA252100.011.9313724
TAGTAGA235900.011.6999676
AGTAGAG269950.09.936317
GTAGAGA311150.09.0965238
TAGAGAC209900.08.9720439
TCGTGTA229400.08.57713174
CGTGTAG203550.08.57619774
TACTCGC48650.08.4413674
CGGAAGA699650.07.75484574
GTCGTGT263900.07.736125574
GTGTAGG212400.07.609406574
GTTCTAG19800.07.47388461
TTGCGTA5500.00101700047.39888052
ATTGCGT9551.00155376E-77.36040641
CACGCGA159950.07.30953814
Produced by FastQC (version 0.11.5)