FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30785391
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA22350.06.949885472
CGATCGT10951.5105434E-76.7574055
GTCGTGT236700.06.74999474
CGTGTAG200300.06.684139374
CGCATAT22150.06.678909373
TCGTGTA214200.06.198609473
GCGTCGT308200.06.120056674
TATTTCG9452.3458852E-45.87289813
CGCCCTA9552.7218965E-45.809077373
ACTCGTA29650.05.49023111
CCTAACG13001.9794212E-55.407205667
ACGTACG9750.0021977165.313526643
TTACTCG29300.05.30346739
GTATCCA128750.05.28703361
TCGATCG14906.423241E-65.214334
GATCGGA727050.05.16829574
CGTCGTG329400.05.16478374
AGGGGGG131600.05.1444231
ATCGGAA709050.05.04391274
TAGAGAC190250.05.01683042
Produced by FastQC (version 0.11.5)