FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35293750
Sequences flagged as poor quality0
Sequence length35-80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGT21951.22036E-86.21406774
TCGTATG24503.2396201E-96.03123874
CGTATGC30259.094947E-126.012067374
CGATCGT20504.1836756E-115.71334845
TAACTCG13100.00264187415.639898374
GTTACCG13150.00276405835.618453574
CCTTTCG110050.05.47412974
ATTGCCG38851.8189894E-125.4126574
CGTATGG18954.9742725E-55.398368474
CTCGTAT15351.8251376E-65.323679469
ACGCTTC70700.05.07343242
Produced by FastQC (version 0.11.5)