FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22067763
Sequences flagged as poor quality0
Sequence length20-80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGA335250.08.9107421
TAGAGAC229400.07.93704032
TAGAGAT173800.07.30655672
CGCATAT16007.1122486E-107.090895773
AGAGACG135100.06.8577023
CGCGACA6200.0030928226.65967259
AGACGGG130350.06.613655
GAGACGG138050.06.47796774
ACGGGGT127100.06.05050567
CGGGGTT125100.05.97603758
GGGGTTT288200.05.6102169
GACGTAT8900.00298868825.58154563
ACCCCGT66450.05.55417474
GTAGCGA9856.476251E-45.4481771
GTTTCAC392900.05.41716512
GCGCATA19402.0478183E-85.346617764
GGTTTCA398650.05.339017411
TTTCACC411600.05.257743413
CGCGTCT12700.00280429075.21115773
TTCACCG56950.05.02446814
Produced by FastQC (version 0.11.5)