FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13282580
Sequences flagged as poor quality0
Sequence length20-80
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGC4850.00822854710.59298574
GTATACG6500.0068408379.03313974
CGCATAT10603.563443E-47.61638574
GCGCATA12503.270911E-56.29226473
ATTGCCG22352.910383E-115.99479172
CGGTAAG9850.00230838785.7042372
AGACGTC18250.00105756615.630244374
GCAATAT26555.474114E-65.52875774
GGTACTC37404.3983164E-95.494756774
ATATAGG20759.235517E-45.305608774
ATGGGCG25102.6413545E-85.287945773
GGCGCAT18600.0080613945.12970674
TCGCGGG20300.0040304045.061672774
Produced by FastQC (version 0.11.5)