FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18508642
Sequences flagged as poor quality0
Sequence length20-80
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGA263150.07.89238071
TAGAGAC185450.07.0561682
AGAGACG103200.07.0211443
ACGGGGT89600.06.7587417
AGACGGG93750.06.72913845
CGGGGTT88300.06.4503148
GAGACGG111200.06.41877174
TGCGATA7557.9071417E-46.237643233
TTCACCG42200.06.06474814
GTCTCGC40850.06.00213581
GGGGTTT213050.05.8884499
AAGTACG8150.0052287965.77182870
GACGGGG107550.05.59780176
TAGAGAT149050.05.56269172
GTTTCAC278350.05.55609112
TCTGTCG37150.05.43392568
GGTTTCA286900.05.31513411
TCACCGT50300.05.30298115
GAGGTAC40754.8567017E-105.219416674
GCGCATA12005.3259276E-45.182236769
Produced by FastQC (version 0.11.5)