FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18508642
Sequences flagged as poor quality0
Sequence length20-80
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGA262550.08.3684181
AGAGACG102500.07.96297033
TAGAGAC182850.07.62976262
AGACGGG97300.07.5757745
ACGGGGT94400.07.00847677
CGGGGTT91850.07.00727758
GAGACGG111950.06.84128954
CGGGTAT6550.00114031956.61141127
AGCGTTA7154.4765105E-46.5374813
GACGGGG109000.06.3997246
TCGGACT18801.7001357E-56.143442674
TAGAGAT150700.06.1080282
GGGGTTT224450.06.02381759
GTTTCAC289400.05.889647512
GCGCATA10652.5205192E-45.84194872
CGCATAT12153.4000567E-45.71457973
GGTTTCA299750.05.698466311
TACGATA7800.00606580825.69498756
ATCGCGG14350.0062282845.681330774
TCTATCG7600.00627799335.67731678
Produced by FastQC (version 0.11.5)