FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32631839
Sequences flagged as poor quality0
Sequence length35-80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT18351.8189894E-126.38118745
CCTTTCG100700.05.983662674
ACGTATG37450.05.767962574
TATGCCG21804.3435102E-75.7366374
CGTCGAC13908.550226E-45.72537474
TCGTATG23501.838227E-65.321639574
TCGAAAC17204.0036175E-45.287887674
CGTAAAC9450.0085766595.1026867
ATTGCCG39258.731149E-115.06896274
CGCATAT16900.00180361945.04539674
GGACTCG263150.05.03318974
CAAATCG24955.7172438E-65.012366374
Produced by FastQC (version 0.11.5)