FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38497882
Sequences flagged as poor quality0
Sequence length20-80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAG581600.06.27251055
TAGTAGA579400.06.18481836
TTTAGTA650800.05.8923034
AGTAGAG636950.05.7499887
ACCGCGC127750.05.58456374
AGAGACG343400.05.4059421
ATTAACG14651.4296802E-65.39220241
TTTTTAG743100.05.392152
TTTTAGT730900.05.3447133
GTAGAGA744500.05.31967548
CGGGGTT316050.05.2828966
ATTTTTA908250.05.2383151
ACGGAGT140250.05.14605331
TAGAGAC536750.05.06426959
Produced by FastQC (version 0.11.5)