FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16600303
Sequences flagged as poor quality0
Sequence length35-155
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG4465250.012.214964145-149
CGGGGGG660300.09.327658145-149
GCGGGGG669100.09.018098145-149
GATCGGG73700.08.122279145-149
GGGGCGG586250.07.9236317145-149
CTGAACG47450.07.9057994145-149
AGGGGGG336250.07.864788145-149
TGGGGGG511650.07.7321606145-149
GGCGGGG686350.07.632433145-149
GGGCGGG630800.07.6086526145-149
TCGGGGG164600.07.4028435145-149
GGGGGGT363050.07.254904145-149
GGGGGCG646500.07.2510395145-149
GAATCGG37650.06.9957314145-149
CGATCGG41450.06.675314145-149
ACAACCG141350.06.625364145-149
GATCGGT29800.06.606605145-149
AACCGCG42550.06.502744145-149
GGTCGGG130150.06.3982773145-149
CAACCGA123550.06.368407140-144
Produced by FastQC (version 0.11.5)