FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23417116
Sequences flagged as poor quality0
Sequence length20-80
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCAT25101.3278623E-106.92423374
GTAGAGA367550.06.4578691
TAGAGAC261000.06.08527662
AGAGACG137350.05.66386463
GCGCATA12408.1961654E-55.54195472
AGACGGG126150.05.53896055
ATTGCCG26254.0527702E-65.347638674
GTTCGGG9900.00291350445.195285345
CGGGGTT122900.05.187278
GAGACGG142850.05.0929364
ACGGGGT122000.05.07783567
ACCGCGC51603.6379788E-125.052274774
Produced by FastQC (version 0.11.5)