FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23066473
Sequences flagged as poor quality0
Sequence length35-80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT37700.07.5670321
CCTTTCG58450.06.433226674
TATCGGG10700.00855176.040074374
GGCTAAT67250.06.0367471
TTTCGAA53700.05.80370669
GTTACCG10754.2860815E-55.783755312
CCGTCTT54300.05.72519732
TCGACGT14509.6152036E-45.672746774
CGGTATA21302.0008883E-115.66929323
CCCGTCT60700.05.6638461
TCGATTT15754.5199657E-45.595566774
GTTATCG15651.827586E-65.58485973
CCGAAAT17957.709298E-55.564393574
CGACGTT19351.899025E-95.48150317
TCCGTTT81000.05.418779418
CTTTCGA57500.05.41666768
AGTCGTC36000.05.38609121
CGTTTGA49000.05.375290420
CCCCTCT551050.05.35617541
TCCTGTC696600.05.3238278
Produced by FastQC (version 0.11.5)