FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31570794
Sequences flagged as poor quality0
Sequence length80
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA73450.06.59980373
TCGTATG27150.06.542217774
CGTCTGA340050.06.49658574
CTCGTAT18701.8189894E-126.33227773
CACGTCT442900.06.1827274
ACGTCTG385100.06.13056874
ACACGTC448500.06.105493573
CGTATGC34800.05.954705773
GCACACG576450.05.71323374
TATCGTA7800.00609259875.69275368
CGGAAGA1004500.05.636312574
AGTCACG142150.05.544779373
GATCGGA1178250.05.536922574
AGATCGG1311200.05.508894474
ATCGGAA1113100.05.5019674
AGAGCAC791900.05.47192574
TATGCCG24001.4551915E-115.396477774
CACACGT571250.05.389527374
ACGCGAC18752.2144377E-85.32863674
TAACGCG12506.112794E-55.32861173
Produced by FastQC (version 0.11.5)