FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31178056
Sequences flagged as poor quality0
Sequence length35-80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG24350.010.22101574
CTCGTAT16250.09.05024574
CGTATGC39000.07.106762474
GATCGGT19406.9485395E-96.706044774
CCTTTCG70000.06.545265774
GCGTAAA8656.358559E-56.45881169
GTCGTAT17552.422512E-66.123740774
TAACTCG12800.00212199665.74478474
CGATCGT14153.0970314E-65.4269145
AATTGCG30302.588422E-95.413718774
TATGCCG24306.7694054E-75.353797474
ACGTATG41450.05.322067774
GGTATCG16100.00101107345.26994174
CGAATGC32953.807145E-95.149987774
CGTATGG16800.0017802835.050359774
GCGTATG22409.190444E-85.00875173
Produced by FastQC (version 0.11.5)