FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14449433
Sequences flagged as poor quality0
Sequence length20-80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGA217950.09.01542951
TAGAGAC151800.08.7472632
AGACGGG82000.08.1192255
AGAGACG86500.07.9047513
CGGGGTT82900.07.72693258
GAGACGG88550.07.68121434
CGCATAT9607.5878174E-47.604889474
ACGGGGT84200.07.56489377
GGCGCAT19058.625648E-86.96797374
GGGGTTT189600.06.8708019
TAGAGAT112950.06.339932
ACCCCGT49500.06.301792674
GGTTTCA259850.06.051371611
GTTTCAC253200.06.03978912
GTTTCGC22050.05.874764412
GACGGGG107350.05.8667456
CGTGTTA24150.05.81247719
GTTAGCC57650.05.808223222
TATGGCG17252.9729784E-45.771374
TTCACCG34750.05.695239514
Produced by FastQC (version 0.11.5)