FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14449433
Sequences flagged as poor quality0
Sequence length20-80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4709.915775E-411.40163774
CTAGCGG6400.00120448879.41971274
CGCATAT9209.307769E-99.06886473
GTAGAGA226100.09.0177381
AGAGACG88600.08.7381663
TAGAGAC156550.08.630112
GAGACGG89300.08.4300424
AGACGGG85050.07.92555575
GGCGCAT20808.712959E-107.40695374
ACGGGGT86550.07.2924697
GCGCATA10951.3269346E-77.243196572
CGGGGTT86300.07.1887568
TAGAGAT118000.06.37771842
GGGGTTT199100.06.1779499
GACGGGG105500.06.08403836
GTTTCGC22000.05.86939212
GTTTCAC267900.05.59650412
TTCACCG35500.05.558807414
GTCTCGC23951.8189894E-125.53582241
GGTTTCA272000.05.524711
Produced by FastQC (version 0.11.5)