FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10351070
Sequences flagged as poor quality0
Sequence length20-80
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT9050.015.93711473
GCGCATA11350.011.784389572
GTAGAGA145700.011.3756631
CGCGTTA2350.00756805110.7132120
TAGAGAC98200.010.4859742
GAGACGG52750.010.3415974
AGAGACG60900.09.4277993
AGACGGG51500.09.4099095
TAGAGAT74100.08.9091852
CGTACTG4850.00266161938.63370773
GGCGCAT15900.08.36002271
ATATAGG17452.4374458E-108.34634874
ACGGGGT53950.08.3187757
TACCGTC8350.00166761987.92834974
CGGGGTT57050.07.74084148
GGGGTTT126500.07.63481249
GCTAATT77500.07.0361671
TCGTACT6650.00208516816.91391472
ATCGCGT5500.0071006146.83533361
GTAGCGA5250.0071204276.83337071
Produced by FastQC (version 0.11.5)