FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14747675
Sequences flagged as poor quality0
Sequence length35-155
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG4379750.011.31763145-149
CGGGGGG606550.08.5963745145-149
GCGGGGG617850.08.537834145-149
TGGGGGG545850.08.337053145-149
GATCGGG63050.08.238891145-149
GGGGCGG548350.08.121143145-149
GGGCGGG590650.08.096952145-149
GGCGGGG640900.07.9567914145-149
AGGGGGG300650.07.738582145-149
GGGGGGT369050.07.4211082145-149
GGGGGCG583700.07.4120464145-149
CTGAACG46100.07.2475944145-149
GGGTGGG628450.06.752316145-149
TATATCG12550.06.607078145-149
AGCGTTA13204.0690746E-46.58892634
CGTCTGA146100.06.5334787145-149
GGTCGGG116950.06.4853644145-149
AGCGCAC121200.06.439047145-149
TCGGGGG135400.06.394166145-149
GTGGGGG728900.06.3604584145-149
Produced by FastQC (version 0.11.5)