FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14747675
Sequences flagged as poor quality0
Sequence length35-155
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGG117150.07.8920503145-149
AAGCGCG154900.07.3614125145-149
CGGGGGG371950.07.0746655145-149
CGGTCGG25950.07.0342994145-149
TCGGGGG112850.06.9323406145-149
TAGCTCG36600.06.9305897145-149
TAGAGCG59750.06.863975145-149
GCGGGGG380050.06.8303175145-149
CGCGTCG122100.06.7178135145-149
GTCGGGG173050.06.6852202145-149
AGGGGGG291900.06.683951145-149
GACGCTT109300.06.66228251
GGGTCGG112200.06.6344404145-149
GGTCGGG105200.06.5801325145-149
GCGCGTC118550.06.5190387145-149
CGATCGG32150.06.341398145-149
ATCGGAC31050.06.337004145-149
TGGGGGG404000.06.331514145-149
CGAGCGG50650.06.3186145145-149
GGGGGGG3373650.06.3123183145-149
Produced by FastQC (version 0.11.5)