FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17096525
Sequences flagged as poor quality0
Sequence length35-80
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGC18700.08.60682674
CCTTTCG51050.07.656666874
TCACGAT48750.07.310442474
CTCGTAT7709.1304726E-56.80809171
CGATAGG11303.8927497E-46.612892674
TAGCGAA15602.8376235E-106.410841563
ACGAAAC17151.2389246E-66.36819174
CGTATGG11100.00229339076.214194374
ACGATAC8750.003312025.91236274
GATAGAC69450.05.876417674
ACACGAT28001.4770194E-95.748130374
CGTATGC16301.5869063E-45.642336474
TTTCGAA36250.05.51774563
ACGATAG46850.05.237983769
CGATAGA48250.05.199547371
TTACGCT10000.00295763985.18901864
ACGATAT21053.931108E-55.188335474
Produced by FastQC (version 0.11.5)