FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17895970
Sequences flagged as poor quality0
Sequence length20-80
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT13700.09.582173
GCGCATA17154.7251888E-85.829427272
ATTGCCG36151.2156306E-85.266993574
AGAACGC26051.4910967E-55.22077974
CGTATGC12900.0037779575.088169673
Produced by FastQC (version 0.11.5)