FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24633514
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG5154.8431684E-710.06096674
CGGACTA4358.299172E-48.50804174
TCGTATG24400.07.432090373
CTCGTAT15000.07.4016271
CGTATGC32250.07.00033774
TATCGTA10453.4171626E-66.374924774
TATGCCG18305.4569682E-116.067209774
CCTAACG9300.00730793045.173165369
AATATCG17651.0018266E-65.032252369
Produced by FastQC (version 0.11.5)