FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32492873
Sequences flagged as poor quality0
Sequence length35-80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTCG140500.08.80266474
TTACGGT6650.0011406566.61127817
GGACTCG208450.05.600491574
GTCGACG14650.00135023485.52289874
TCGTGCG8850.00435507675.406521355
AACGTAG10000.00287926455.200217770
Produced by FastQC (version 0.11.5)