FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33772629
Sequences flagged as poor quality0
Sequence length80
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG407750.06.334511874
ACACGTC475700.06.26201974
CGTCTGA348550.06.24257774
CACGTCT459700.06.101637474
CGGAAGA1064050.05.856413474
ATCGGAA1202400.05.8503974
TCGTATG26750.05.809953773
AGAGCAC824550.05.789277674
GCACACG597200.05.737766374
TCGGAAG1202500.05.683730674
AGATCGG1428950.05.67642974
GATCGGA1287650.05.664224674
TAGATCG482900.05.524967774
CCTAATC71400.05.44180274
AGCACAC765800.05.349139774
CAGATCG546000.05.34731874
GTCTGAA385200.05.341216674
AGCGTTA12556.5308835E-55.3063710
CACACGT618850.05.273936374
CTCGTAT15502.3619414E-65.25209372
Produced by FastQC (version 0.11.5)