FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33772629
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG307700.07.54161174
GTCGTGT383750.07.387618574
TCGTGTA342900.07.05895173
GCGTCGT489650.06.764899774
AGAGCGT697450.06.61194374
TCGTGTT40650.06.282212374
TAGGGAA267150.06.220356574
AAGAGCG826450.06.090408374
AGCGTCG622700.06.008919274
GAGCGTC699900.05.964818574
CGTCGTG527800.05.93933974
CGGAAGA1057900.05.870450574
TCGGAAG1165200.05.7586674
ATCGGAA1204450.05.752295574
GTGTAGG373900.05.691627574
GATCGGA1266600.05.677503674
TAGATCG488200.05.66300574
AGATCGG1408500.05.659954574
CAGATCG518900.05.442080574
GAAGAGC1070650.05.161030374
Produced by FastQC (version 0.11.5)