FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16381512
Sequences flagged as poor quality0
Sequence length80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG3100.00433388079.54837113
CGCGAAA4256.602405E-48.70597516
GTCGTGT151350.06.64689774
CGCATAT11051.2682285E-66.35960973
TCGTGTA136150.06.16652774
GATCGCG9051.3002614E-46.1319311
ACGCTTC51850.06.0647852
GACGCTT70000.06.02505971
GCGCATA12401.4263533E-65.96541672
GCGTCGT194800.05.94275574
TGCGATT17709.604264E-105.850826372
CGTGTAG123650.05.77292174
CGTCGCC9300.00121405565.56945756
GTATCCA88750.05.54418661
TAGATCG198500.05.533862674
GGTACCG8050.0086465925.51492931
TAGGGAA124800.05.453002574
CGTCGTG197200.05.382789674
GAGACGG50100.05.31699854
GCGCGGA11254.2774173E-45.263227555
Produced by FastQC (version 0.11.5)