FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30389841
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAA47750.08.21470374
CGTCTGA310600.06.96969574
ACACGTC419150.06.96573474
GCACACG525550.06.435636574
ACGTCTG346650.06.276873
CACGTCT403850.06.185047674
ACGTCGT64800.06.167484874
AGAGCAC742200.05.813490474
TCGTAGA66200.05.813364573
CACACGT516300.05.79827273
GATCGGA1151950.05.76299474
CGGAAGA957150.05.756705874
TCGGAAG1069850.05.72174
ATCGGAA1080550.05.67804774
AGATCGG1283200.05.50805674
AGCACAC697650.05.47926274
AGTCACG105050.05.424803374
AAGATCG274650.05.402864574
GTCTGAA361900.05.26596574
CAGATCG419850.05.23542174
Produced by FastQC (version 0.11.5)