FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30389841
Sequences flagged as poor quality0
Sequence length80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT343950.08.12446974
CGTGTAG274500.07.29461873
GCGTCGT446450.07.22085674
TCGTGTA302300.07.125711474
CGTCGTG436750.06.63554373
AGAGCGT635800.06.49661374
GTGTAGG337100.06.24736574
AAGAGCG751550.06.200278374
AGCGTCG580350.06.05228874
GAGCGTC619450.05.86146474
TAGGGAA251450.05.681709374
TCGGAAG1057850.05.67855474
GATCGGA1155850.05.66140374
CGGAAGA979100.05.5834373
ATCGGAA1099600.05.54373274
AGATCGG1290250.05.44746574
TAGATCG403350.05.340528574
TCGTGTT39700.05.03438774
GAAGAGC984700.05.02540274
Produced by FastQC (version 0.11.5)