FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22417903
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14101.3278623E-96.561872572
ACGATAC11105.8275822E-55.66811574
TCGTATG17907.510607E-95.582232573
ACGCTTC72950.05.57971862
CGATAGA98350.05.569094773
GACGCTT86000.05.46588661
ACAATCG13809.318412E-65.363689474
ACGAAAC21252.6375346E-95.224864574
CGTATGC27851.8189894E-125.18246873
GATAGAC113050.05.107010474
GTCGTAT10900.00159248575.093044874
CGATCGT10250.0041185325.05303418
ACGATAG106900.05.01992572
Produced by FastQC (version 0.11.5)