FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25180587
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19550.07.19380674
CTCGTAT16802.6375346E-106.16804271
TCGTATG23700.06.089966371
CGGATTG78700.05.78413673
GCGTATG21501.8189894E-115.68048472
CGATCGT10952.9193287E-45.4068155
ACGGATT21405.657057E-105.361296774
CAAATCG14151.44131645E-55.23112974
CCGGATT86250.05.063289672
Produced by FastQC (version 0.11.5)