FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14619051
Sequences flagged as poor quality0
Sequence length35-155
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG3438250.011.154658145-149
CGGGGGG514150.09.092448145-149
GCGGGGG535200.08.459682145-149
GGGGCGG470600.08.284418145-149
GGGCGGG518250.07.975426145-149
GATCGGG66300.07.6421475145-149
GGCGGGG566050.07.5576878145-149
TGGGGGG427150.07.4793262145-149
GGGGGGT291450.07.4162908145-149
AGGGGGG266850.07.3236485145-149
GGGGGCG518300.07.2474995145-149
GGTCGGG107150.06.9881463145-149
CTGAACG46700.06.8945475145-149
CGTCTGA148500.06.822415145-149
GATCGTA18950.06.322152145-149
GCGCGGG190100.06.262809145-149
GATCGGT26650.06.1812973145-149
TATATCG11751.0004442E-106.1601753145-149
ACACGTC160850.06.153915140-144
CAGATCG109100.06.0230055135-139
Produced by FastQC (version 0.11.5)