FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14619051
Sequences flagged as poor quality0
Sequence length35-155
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGG93550.08.126085145-149
AAGCGCG127100.07.8392797145-149
CGATCGG26950.07.39423145-149
ATCGGAC27900.07.3188114145-149
TAGAGCG46000.06.952679145-149
CGGGGGG300200.06.924887145-149
TCGGGGG94200.06.842536145-149
GTCGGGG132800.06.7432613145-149
CCGATCG12100.06.7095785145-149
GATCGTA36150.06.6257863145-149
TAGCTCG32350.06.6162457145-149
GATCGGT24550.06.6139226145-149
GGGTCGG86800.06.5472503145-149
CGCGTCG116700.06.514098145-149
CGGTTAG8755.4927295E-76.4363847145-149
AGCTCGG136800.06.420203145-149
GCGGGGG311250.06.4182014145-149
CGGTCGG22700.06.394297145-149
GGGGGGT251300.06.392745145-149
GATCGGG55950.06.3757987145-149
Produced by FastQC (version 0.11.5)